The identity of the Scarlet Spider has been used by several characters: Ben Reilly, Peter Parker (while Ben posed as him in prison), Joe Wade (a villain), and a group of three Michael Van Patrick (MVP) clones working with the Initiative.
as Ben Reilly-
The first Scarlet Spider is a clone of Spider-Man created by the Jackal. The Jackal creates the clone to fight and defeat the original Spider-Man, but since the clone and Peter both share the same memories, they confuse themselves in the battle. The winner is the original Peter Parker. The clone is left for dead and is dumped into a smoke stack of a factory. He escapes and assumes the alias of Ben Reilly. The name is a combination of his Uncle's first name and his Aunt's maiden name. He wanders around aimlessly for five years. He returns to Spider-Man's home of New York, and finds himself once again wanting to be a hero. He fashions his own costume, and becomes the Scarlet Spider. He fights alongside his "brother" Spider-Man, and proves himself to be such a great hero, that Spider-Man asks him to watch over the city, while he and his wife try to start a family. When his name is smeared by a second, evil Scarlet Spider, he assumes the identity of Spider-Man, until his death at the hands of Norman Osborn. His body disintegrates, proving once and for all that he is the clone.
as Joe Wade (villain)
An undercover FBI agent assigned to investigate the new Doctor Octopus, Carolyn Trainer, Joe Wade is discovered and forced to undertake a virtual reality graft. Carolyn turns him into a hard-light holographic duplicate of the Scarlet Spider, in order to ruin his name. In his Scarlet Spider guise, Joe is actually trapped in a virtual reality chamber, and his thoughts power the hologram. Despite this, Joe is unable to stop himself from committing acts of violence. When the real Scarlet Spider, Ben Reilly, attacks Doctor Octopus's lair, he damages the machine while Joe is still inside. The damage to the virtual reality chamber causes the grafts to malfunction, and Joe becomes a real mechanized Scarlet Spider with amazing powers. As the new Scarlet Spider, Joe is superhumanly strong and fast, and has claws on his fingertips; he can also fire webbing from his wrists, crawl up walls, and fire laser 'stingers' from his eyes. It takes both Ben Reilly (in the guise of Spider-Man) and the New Warriors to stop the cybernetic Scarlet Spider, and the FBI put him in custody and have him undergo medical treatments to remove the technology.
the Scarlet Spiders-
The three clones, Michael, Van, and Patrick (sometimes referred to as "Pat"), were each suited up in redesigned Iron Spider Stark armor which Spider-Man had worn during the early stages of the superhero Civil War.
During their first appearance, they identify themselves as "Red Team" and take down supervillains Shocker, Boomerang, and Hydro-Man. Though War Machine identifies them by their official name Scarlet Spiders when contacting them, they still identify themselves as Red Team One (Michael), Red Team Two (Van), and Red Team Three ("Pat" Patrick) in the field. They are later revealed to be part of a black ops group called the Shadow Initiative under the command of Henry Gyrich with Constrictor, Mutant Zero, Bengal and Trauma.
The Scarlet Spiders were forced to expose themselves to the public in Avengers: The Initiative #7 after an attack on Baron Von Blitzschlage. The subsequent pursuit of three criminals wearing the Vulturions flying exosuits would lead the spiders into confronting an angry Peter Parker. Their appearance in battle against and alongside Parker has raised public doubts over whether Parker is the original and/or only Spider-Man, despite his having publicly revealing his dual identity in the early events of the Civil War.
Now public, the Scarlet Spiders join with the other initiative trainees in the next issue. An incident involving Dragon Man leads one of the Spiders to save Cloud 9 in much the same fashion as the original MVP did at the time of his death. In Avengers: The Initiative #10, the Scarlet Spiders unmask themselves in an attempt to calm the enraged MVP clone, KIA, only to have him behead one of their number. The surviving (and still unmasked) Scarlet Spiders, Michael and Patrick[, are later seen by the majority of the trainees, including Cloud 9. In Avengers: The Initiative #11, the remaining Scarlet Spiders make there way to Bulls Gap, Tennessee to aid in the capture of KIA. In Avengers: The Initiative #12 after the defeat of KIA, the surviving Scarlet Spiders, along with the MVP clone and the real MVP's father, join with Justice's New Warriors as a Counter Initiative of underground registered heroes